I-SceI in complex with a top nicked DNA substrate

PDB ID: 3C0X
Gene source organism: Saccharomyces cerevisiae
Expression organism: Escherichia coli BL21(DE3) Deposition date: 2008-01-21
Classification: hydrolase/DNA
Keywords: endonuclease, homing, LADLIDADG, catalytic mechanism, metal binding, nicked intermediate, Hydrolase, Intron homing, Mitochondrion, mRNA processing, mRNA splicing, hydrolase-DNA COMPLEX
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.3
R-value: 0.255
Metal site idBound amino acidsBound ligands IDs
3C0X-CA-1 C1T1 DC C.15 DT D.16 HOH C.433 HOH D.412 HOH D.432
3C0X-CA-2 A1C1D2 ASP A.144 ASP A.44 DA B.14 DC D.17 HOH A.428 HOH D.435
3C0X-CA-3 C2D2 ASP A.145 ASP A.44 DC C.15 DC D.17 HOH A.425