I-MsoI re-designed for altered DNA cleavage specificity (-8G)

PDB ID: 3MIS
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2010-04-12
Classification: DE NOVO PROTEIN/DNA
Keywords: protein-DNA complex, homing nuclease, Rosetta design, DE NOVO PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.3
R-value: 0.2456
Metal site idBound amino acidsBound ligands IDs
3MIS-CA-1 C1D1G2 ASP A.22 DC D.2 DG C.3 GLY B.21 HOH B.4
3MIS-CA-2 A1D1G2 ASP B.22 DA C.2 DG D.3 GLY A.21 HOH B.5 HOH D.48
3MIS-CA-3 C1D2G2 ASP A.22 ASP B.22 DC D.2 DG C.3 DG D.3