Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with a 5'-Phosphorylated 14-mer/12-mer Duplex and Magnesium

PDB ID: 3SM4
Gene source organism: Enterobacteria phage lambda
Expression organism: Escherichia coli Deposition date: 2011-06-27
Classification: HYDROLASE/DNA
Keywords: homologous recombination, DNA repair, recombineering, single-strand annealing, Type II Restriction Endonuclease Fold, 5'-3' dsDNA exonuclease, HYDROLASE-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 1.88
R-value: 0.15976
Metal site idBound amino acidsBound ligands IDs
3SM4-MG-1 D1E1G1L1 ASP B.119 DG E.2 GLU B.129 HOH B.550 HOH E.543 LEU B.130
3SM4-MG-2 A1D1G1 ASP B.119 DA E.1 DG E.2 HOH B.277 HOH B.305 HOH B.310