Crystal structure of the mutant D75N I-CreI in complex with its wild- type target in presence of Ca at the active site (The four central bases, 2NN region, are composed by GTAC from 5' to 3')

PDB ID: 4AAG
Gene source organism: CHLAMYDOMONAS REINHARDTII
Expression organism: ESCHERICHIA COLI Deposition date: 2011-12-01
Classification: HYDROLASE/DNA
Keywords: HYDROLASE-DNA COMPLEX, GENE TARGETING, PROTEIN-DNA INTERACTION, HOMING ENDONUCLEASES
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.8
R-value: 0.1735
Metal site idBound amino acidsBound ligands IDs
4AAG-CA-1 C1D1G2 ASP A.20 DC E.514 DG G.615 GLY B.19 HOH A.2007 HOH A.2018
4AAG-CA-2 C1D1G2 ASP B.20 DC G.614 DG E.515 GLY A.19 HOH B.2001 HOH B.2015