Crystal structure of the homing endonuclease I-CvuI in complex with I- CreI target (C1221) in the presence of 2 mM Mg revealing DNA not cleaved

PDB ID: 5A78
Gene source organism: CHLORELLA VULGARIS
Expression organism: ESCHERICHIA COLI Deposition date: 2015-07-03
Classification: HYDROLASE/DNA
Keywords: HYDROLASE-DNA COMPLEX, GENE TARGETING, GENETICS, PROTEIN-DNA INTERACTION, HOMING ENDONUCLEASES
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.5
R-value: 0.2151
Metal site idBound amino acidsBound ligands IDs
5A78-MG-1 A1C1D1G1 ALA B.22 ASP A.23 DC C.514 DG D.615 HOH A.2009
5A78-MG-2 A1C1D1 ALA A.22 ASP B.23 DC D.614