Engineered variant of I-OnuI meganuclease targeting the Human TCRa gene; harbors 43 point mutations relative to wild-type I-OnuI

PDB ID: 5T2H
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2016-08-23
Classification: HYDROLASE/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, Homing Endonuclease, HYDROLASE-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.52
R-value: 0.2116
Metal site idBound amino acidsBound ligands IDs
5T2H-CA-1 A2C1E1 ALA A.21 DA C.16 DC B.14 GLU A.178 HOH A.504
5T2H-CA-2 A1E1G1T1 DA B.15 DT C.15 GLU A.22 GLY A.177 HOH A.515 HOH A.519