Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP007280 gene; harbors 38 point mutations relative to wild-type I-OnuI

PDB ID: 5T2N
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2016-08-23
Classification: Hydrolase/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, Homing Endonuclease, Hydrolase-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.08
R-value: 0.2199
Metal site idBound amino acidsBound ligands IDs
5T2N-CA-1 A1E1G1T1 DA C.15 DT B.15 GLU A.22 GLY A.177 HOH A.507 HOH A.533
5T2N-CA-2 A1C1E1G1 ALA A.21 DC B.14 DG C.16 GLU A.178 HOH A.508 HOH B.107
5T2N-CA-3 A1C1E2G1T1 DA C.15 DC B.14 DG C.16 DT B.15 GLU A.178 GLU A.22