Engineered variant of I-OnuI meganuclease targeting the Anopheles AGAP011377 gene; harbors 53 point mutations relative to wild-type I-OnuI

PDB ID: 5T2O
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2016-08-23
Classification: Hydrolase/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, Homing Endonuclease, Hydrolase-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.8
R-value: 0.2243
Metal site idBound amino acidsBound ligands IDs
5T2O-CA-1 A1C1E1T1 ALA A.21 DC B.14 DT C.16 GLU A.178 HOH C.103
5T2O-CA-2 A1E1G1T1 DA C.15 DT B.15 GLU A.22 GLY A.177