Engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 47 point mutations relative to wild-type I-OnuI

PDB ID: 5T8D
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2016-09-07
Classification: HYDROLASE/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, Homing Endonuclease, HYDROLASE-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.15
R-value: 0.1824
Metal site idBound amino acidsBound ligands IDs
5T8D-CA-1 A1E1G1T1 DA B.17 DT C.15 GLU A.22 GLY A.177 HOH A.566 HOH A.619
5T8D-CA-2 A2C1E1 ALA A.21 DA C.16 DC B.16 GLU A.178 HOH A.540 HOH B.145
5T8D-CA-3 A2C1E2T1 DA B.17 DA C.16 DC B.16 DT C.15 GLU A.178 GLU A.22