Engineered variant of I-OnuI meganuclease targeting the HIV CCR5 gene; harbors 43 point mutations relative to wild-type I-OnuI

PDB ID: 5THG
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2016-09-29
Classification: HYDROLASE/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, HYDROLASE-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 3.1
R-value: 0.2206
Metal site idBound amino acidsBound ligands IDs
5THG-CA-1 E1G1T2 DT B.18 DT C.17 GLU A.22 GLY A.177 HOH C.101
5THG-CA-2 A1C1E2T1 ALA A.21 DC B.17 DT C.18 GLU A.178 GLU A.22 HOH A.501