Crystal structure of an aminoglycoside acetyltransferase meta-AAC0020 from an uncultured soil metagenomic sample in complex with sisomicin

PDB ID: 5U08
Gene source organism: uncultured bacterium
Expression organism: Escherichia coli BL21(DE3) Deposition date: 2016-11-23
Classification: TRANSFERASE/ANTIBIOTIC
Keywords: GNAT fold, GCN5-N-acetyltransferase fold, acetyltransferase, aminoglycoside, sisomicin, antibiotic resistance, metagenome, soil, coenzyme A, structural genomics, Center for Structural Genomics of Infectious Diseases, CSGID, TRANSFERASE-ANTIBIOTIC complex
Technique: X-RAY DIFFRACTION
Assembly: 3
Resolution: 1.52
R-value: 0.1659
Metal site idBound amino acidsBound ligands IDs
5U08-CA-1 D1E1F1 ASP A.44 GLU B.83 HOH A.471 HOH B.469 HOH D.355 PHE D.40
5U08-CA-2 D1E1F1 ASP D.44 GLU C.83 HOH A.361 HOH C.324 HOH C.427 PHE A.40