Original engineered variant of I-OnuI meganuclease targeting the HIV integrase gene; harbors 49 point mutations relative to wild-type I-OnuI

PDB ID: 5V0Q
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2017-02-28
Classification: HYDROLASE/DNA
Keywords: Meganuclease, Engineered protein, DNA complex, Homing Endonuclease, HYDROLASE-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.4
R-value: 0.1856
Metal site idBound amino acidsBound ligands IDs
5V0Q-CA-1 A1E1G1T1 DA B.15 DT C.15 GLU A.22 GLY A.177 HOH A.508 HOH B.112
5V0Q-CA-2 A2C1E1 ALA A.21 DA C.16 DC B.14 GLU A.178 HOH A.509 HOH B.111