Crystal structure of Cas12i2 ternary complex with double Mg2+ bound in catalytic pocket

PDB ID: 6LTU
Gene source organism: unidentified
Expression organism: Escherichia coli 'BL21-Gold(DE3)pLysS AG' Deposition date: 2020-01-23
Classification: HYDROLASE/RNA/DNA
Keywords: CRISPR-Cas, Cas12i2, Cas12i2 ternary complex, HYDROLASE, HYDROLASE-RNA-DNA complex, active RuvC state, wild type Cas12i2, wt Cas12i2
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.57
R-value: 0.202
Metal site idBound amino acidsBound ligands IDs
6LTU-MG-1 C1D2N1 ASN A.601 ASP A.1019 ASP A.599 DC E.24 HOH A.1253 HOH E.101
6LTU-MG-2 C2D1E1 ASP A.599 DC E.23 DC E.24 GLU A.833 HOH A.1209 HOH A.1242