Engineered variant of I-OnuI meganuclease with improved thermostability and fully altered specificity targeting cholera toxin A subunit

PDB ID: 6UW0
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2019-11-04
Classification: DNA BINDING PROTEIN/DNA
Keywords: Meganuclease, Homing Endonuclease, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 2
Resolution: 2.7
R-value: 0.2234
Metal site idBound amino acidsBound ligands IDs
6UW0-CA-1 E1G1T2 DT C.15 DT D.15 GLU B.22 GLY B.177 HOH B.502
6UW0-CA-2 A1C1E1G1 ALA B.21 DC C.14 DG D.16 GLU B.178 HOH B.503