Engineered variant of I-OnuI meganuclease with improved thermostability and E178D mutation at catalytic site

PDB ID: 6UWG
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2019-11-05
Classification: DNA BINDING PROTEIN/DNA
Keywords: Meganuclease, Homing Endonuclease, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.22
R-value: 0.1932
Metal site idBound amino acidsBound ligands IDs
6UWG-CA-1 A2C1D1 ALA A.21 ASP A.178 DA C.16 DC B.14 HOH A.517 HOH A.541
6UWG-CA-2 A1E1G1 DA B.15 GLU A.22 GLY A.177 HOH A.543 HOH A.550 HOH C.102
6UWG-CA-3 A1E1T2 DA C.16 DT C.15 GLU A.22 HOH A.518 HOH C.112 THR A.48
6UWG-CA-4 A3D1E1 ALA A.21 ASP A.178 DA B.15 DA C.16 GLU A.22