Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity

PDB ID: 6UWJ
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2019-11-05
Classification: DNA BINDING PROTEIN/DNA
Keywords: Meganuclease, Homing Endonuclease, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 1.85
R-value: 0.1909
Metal site idBound amino acidsBound ligands IDs
6UWJ-CA-1 A2C1D1 ALA A.21 ASP A.178 DA C.16 DC B.14 HOH A.511 HOH A.565
6UWJ-CA-2 E1G2 DG B.15 GLU A.22 GLY A.177 HOH A.553 HOH A.572 HOH C.204
6UWJ-CA-3 A1E1T2 DA C.16 DT C.15 GLU A.22 HOH A.508 HOH C.216 THR A.48