Engineered variant of I-OnuI meganuclease with improved stability and fully altered specificity targeting human chromosome 11 trans integration site

PDB ID: 6UWK
Gene source organism: synthetic construct
Expression organism: Escherichia coli Deposition date: 2019-11-05
Classification: DNA BINDING PROTEIN/DNA
Keywords: Meganuclease, Homing Endonuclease, DNA BINDING PROTEIN, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.53
R-value: 0.2334
Metal site idBound amino acidsBound ligands IDs
6UWK-CA-1 A2C1D1 ALA A.21 ASP A.178 DA C.16 DC B.14 HOH A.508 HOH A.510
6UWK-CA-2 C1I1 DC B.6 HOH B.108 HOH B.109 ILE A.69
6UWK-CA-3 E1G2 DG B.15 GLU A.22 GLY A.177 HOH A.512 HOH C.102
6UWK-CA-4 A1E1T1 DA C.16 GLU A.22 HOH A.514 HOH C.101 THR A.48