First stage engineered variant of I-OnuI after initial reassembly

PDB ID: 7RCC
Gene source organism: Synthetic construct
Expression organism: Escherichia coli Deposition date: 2021-07-07
Classification: DNA BINDING PROTEIN/DNA
Keywords: meganuclease, gene editing, protein engineering, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.45
R-value: 0.2219
Metal site idBound amino acidsBound ligands IDs
7RCC-CA-1 C3D1E1 ASP A.178 DC E.14 DC E.15 DC F.16 GLU A.22
7RCC-CA-2 C1E1G1T1 DC E.15 DT F.15 GLU A.22 GLY A.177
7RCC-CA-3 A1C2D1 ALA A.21 ASP A.178 DC E.14 DC F.16 HOH F.103