Second stage reengineered variant of I-OnuI targeting human PD1 gene with activity enhancing substitutions

PDB ID: 7RCD
Gene source organism: Synthetic construct
Expression organism: Escherichia coli Deposition date: 2021-07-07
Classification: DNA BINDING PROTEIN/DNA
Keywords: meganuclease, gene editing, protein engineering, DNA BINDING PROTEIN-DNA complex
Technique: X-RAY DIFFRACTION
Assembly: 1
Resolution: 2.448
R-value: 0.2354
Metal site idBound amino acidsBound ligands IDs
7RCD-CA-1 A1C2D1 ALA A.21 ASP A.178 DC B.14 DC C.16 HOH A.502 HOH B.102
7RCD-CA-2 C1E1G1T1 DC B.15 DT C.15 GLU A.22 GLY A.177 HOH A.505