SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state

PDB ID: 7Z4J
Gene source organism: Streptococcus pyogenes
Expression organism: Escherichia coli Deposition date: 2022-03-03
Classification: HYDROLASE
Keywords: CRISPR, Cas9, R-loop, substrate binding, off-target, HYDROLASE
Technique: ELECTRON MICROSCOPY
Assembly: 1
Resolution: None
R-value: None
Metal site idBound amino acidsBound ligands IDs
7Z4J-MG-1 A1D1E1T1 ASP B.10 DA D.-4 DT D.-3 GLU B.762
7Z4J-MG-2 D1G1N1T1 ASN B.863 ASP B.839 DG c.3 DT C.4 HOH B.1501 HOH C.101